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In addition, the total of 14, 24, 37 and 27 mutations were successfully chosen at 1, 3, 10 and 20% of MnCl2.
Collectively, Southern and Western blot analysis indicated that the intended mutations were successfully introduced into the HSV-2 ICP0 gene.
Once the mutations were successfully incorporated, the 1.7 kB hCE1 cDNA was ligated into pCIneo for mammalian cell expression (Promega, Madison, WI).
KRAS and BRAF mutations were successfully evaluated in 525 and 524 cases, respectively.
Using the SureSeq panel, all of the mutations were successfully detected.
Using the Fluidigm panel, 20 of the 24 mutations were successfully detected.
Similar(50)
Several causative genes for juvenile ALS or ARJALS have been reported (Hadano et al., 2001; Chen et al., 2004; Meyer et al., 2005; Chow et al., 2009; Wijesekera and Leigh, 2009), although mutations are successfully identified in only a fraction of the patients.
Based on previous experience [ 29], two patients of our cohort with trisomy 21 mosaic and GATA1 mutation were successfully treated with a reduced intensity regimen for DS-AMKL paccordingaccording to treatment recommendation of the ML-DS 2006 registry).
Repair of a splicing defect of β-globin pre-mRNA harboring hemoglobin E (HbE) mutation was successfully accomplished in erythroid cells from patients with β-thalassemia/HbE disorder by a synthetic splice-switching oligonucleotide (SSO).
One newly designed and easily synthesized phosphorylated BChE nonapeptide with one Gly-to-Ala mutation was successfully reported to serve as internal standard instead of traditional isotopically labeled BChE nonapeptide.
A control sample with this mutation was successfully analysed, confirming the negative result of this mutation in the described series.
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CEO of Professional Science Editing for Scientists @ prosciediting.com