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Combinatorial mutations were subsequently introduced, and the superior mutant was double mutant E161D/N365P.
All mutations were subsequently verified by sequencing (Australian National University, Biomolecular Resource Facility).
Similar observations of heterozygous mutations were subsequently made in human tumors [ 18].
The corresponding mutations were subsequently manually added to the obtained network.
Many more inactivating mutations were subsequently found, accounting for ∼25% of FGD cases.
PCR products were amplified from genomic DNA isolated from tail biopsies, analyzed for RFLPs, and mutations were subsequently confirmed by Sanger sequencing.
Similar(38)
A series of stabilizing mutations are subsequently combined and result in a doubling of the ΔG value of unfolding.
The identified mutations are subsequently re-engineered into a reference wild-type molecular clone, and the resulting replication capacity and level of drug resistance are determined relative to the wild-type virus.
Pseudogenes had one or more mutations that would prevent correct translation; each of the inactivating mutations was subsequently checked against the original sequencing data.
The presence of slightly deleterious mutations was subsequently identified as the likely reason for the excess of nonsynonymous polymorphism, compared with divergence, in mitochondrial protein-coding genes (Nachman 1998; Rand and Kann 1998; Gerber et al. 2001).
The DNA fragments between −450 and −206 of RETN with or without this point mutation were subsequently cloned into a luciferase reporter vector pGL4.11 (Promega) at the site between Kpn I and Bgl II.
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