Theoretically, phylogenetic reconstructions based on different housekeeping genes must result in concordant trees in a clonal population in which accumulation of mutations is solely responsible for genetic diversification.
Because the Ser310 alleles are functional, while A40V is not, it is unlikely that the cause of the malfunction of the A40V mutation is solely reduced BicD phosphorylation.
Thus, we can conclude that this single amino acid mutation is solely responsible for the increase in virulence of the QA23ML-3Egg vinus in mice.
Thus, the differences between 3D reconstructions for actin filaments decorated with wild-type smooth muscle S1 and those containing the R403Q mutation are solely due to changes in variability in the entire myosin region and not due to partial decoration.
No single mutation was solely responsible for the improvements in tolerance, and all five were needed to reproduce the levels observed in evolved strains.
In order to test whether this mutation is solely responsible for all observed differences in transcript levels of Iogen-M4 and Iogen-M8, we performed transcript analysis of Iogen-M4X.
The breadth of the WNV CP40 mutant swarm was shown to be directly correlated with virus adaptation, and reverse genetics studies demonstrated that consensus mutations were not solely responsible for the adaptive phenotype, indicating the importance of mutant swarm variants in host adaptation and overall viral fitness [ 19, 22].
Thus, the toxicity of FUS mutations may be solely due to their impact on nuclear import [ 6, 13, 17, 22, 26].
They were solely responsible.
An important consideration in any type of random mutagenesis screen is the impact that mutation load and "off-target" mutations will have on the ability to determine that a specific mutation of interest is solely responsible for the observed mutant phenotype.
