Interestingly, when Q523-L and I769-V mutations were separately introduced into S EP3), both mutants showed a weak cell cell fusion activity in Vero cells (Fig. 5b panel EP3, EP3(Q523-L) and EP3(I769-V)).
As for PML-RARA, indirect evidence from mouse models suggests that mutated NPM1 and fusion proteins of CBFB, RUNX1 and MLL are initiating mutations for AMLs (reviewed in McCormack et al).. AML mutations were separately analyzed in each of the AML subgroups.
This can be detected either qualitatively, if the same mutations are separately detected in two different libraries, or quantitatively (if the two strains are in the same library, their shared mutations will be observed on average at double the expected read count).
Given that dovitinib may exert its anticancer effects either by directly targeting FGFRs or regulators of angiogenesis, endometrial cancer patients with or without FGFR2 mutations are separately enrolled in a clinical to prove its mechanisms of functions [ 166].
For production of pure and homogenenous bispecific antibodies, anti-FGFR1 and anti-KLB arms with the knob or the hole mutation were separately purified from transiently or stably transfected CHO cell culture supernatant by affinity purification using Protein A column, and then subjected to an annealing protocol as previously described (Shatz et al., 2013).
Losses of mutation classes and fixations of mutations were recorded separately.
Mutations were introduced separately in rad8A or rad8B-noXhoI containing plasmids prior to generation of the full gene.
However, when types of mutations were analysed separately for each gene, KRAS codon 12 mutations were predictive for increased risk of death (HR 1.62, p=0.014).
The following mutations were incorporated separately into pKW11: UUG, UCG, UAG, UGG, or UGU as the -1 triplet, and the additional mutants U85A, and A86C.
KIR and KID mutations were introduced separately into the mouse genome by a knock-in technique (Fig. 1); therefore, the mutated IGF1 molecule is expressed under its natural promoter (and the regulation of the Igf1 promoter is not affected).
The mutations were introduced separately into a given subunit of the trimers; in the central position to get a single mutant; in the first and the last subunits to get a double mutant and in all three subunits to get a triple mutant.
