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To detect potential novel gene defects that may contribute to hereditary BC susceptibility, 143 patients belonging to 143 Chilean families tested for BRCA1 and BRCA2 mutations were screened for mutations in RAD51, using conformational sensitive gel electrophoresis (CSGE) and DNA sequencing.
Mutations were screened by direct sequencing of the PCR products with specific primers (Table S2).
In exon 11 of the B-RAF gene and in exon 1 of the N-RAS gene mutations were screened by 'radio-active' SSCP technique.
The double mutant control mutations were screened in order to maintain the same notional melting temperature (Tm) as the wild-type Tm.
Suppressor mutations were screened based upon their ability to restore viability to the tpk null strain and were more readily identified from the prkaca-F327A background than from the prkaca-L173A background (Tables 1 and 2).
KRAS and BRAF mutations were screened using current protocols.
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The presence of the desired mutations was screened initially by the gain or loss of a restriction site and was confirmed by DNA sequencing.
The presence of mtDNA mutations was screened by temporal temperature gradient electrophoresis (TTGE).
As the next step in TILLING, the genomic DNA from these animals containing induced mutations is screened with discovery technologies.
In conclusion, our data showed that NPD is useful for CF diagnosis when classes I-III CFTR mutations are screened.
The presence of mitND6 gene mutations was screened by DNA sequencing of tumor tissues from 87 primary lung adenocarcinoma patients and the correlation of the mutations with the clinical features was analyzed.
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mutations were observed
mutations were confirmed
mutations were created
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