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These three missense mutations were predicted to be damaging in functional prediction by SIFT and PolyPhen2.
All mutations were predicted as not tolerated by the SIFT prediction program and predicted as probably damaging by PolyPhen2, except for p.Ala284Asp (c.851C > A), which was predicted as possibly damaging.
Similarly, NRAS Q61K/L/R mutations were predicted to generate neoantigens in the presence of certain HLA-A alleles.
Antigenic-index alterations following L1 mutations were predicted by Jameson Wolf algorithm.
Logistic regression showed that K-ras G --> T transversion mutations and p53 exon 6 mutations were predicted by the country of residence of the patients.
In an additional challenge, steady state gene levels in response to double knockout mutations were predicted.
In the HGMD dataset, more missense mutations were predicted to be deleterious by the SVMpolynomial combination than nsSNPs from dbSNP.
SNAP predicted only 1731 (11.3%) of these mutants to be neutral, so the great majority of mutations were predicted to be non-neutral.
In the HGMD dataset, the majority of missense mutations were predicted to be deleterious by SIFT (65.2%) and PANTHER (70.6%), and to be damaging by Polyphen (73.6%).
All mutations were predicted to be inviable.
Most disease-causing mutations were predicted to impact protein function.
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