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If two or more of these mutations were picked up by selection and simultaneously started to increase in frequency, at some point they started to interfere with each other, thus preventing the fixation of any allele and the sweep itself.
Actually, the fact that ESX-1 mutations were picked up supports the reliability of our screen.
These mutations were picked up in half (49%) of cases in our study.
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Only a fraction of the many possible mutations are picked up by expanded newborn screening, and precious days are lost while test results are processed.
To reduce the likelihood of fixing additional mutations during this process, colonies containing the secondary mutation were picked and restreaked as soon as they were visible (approximately 14 hours).
The masks used in mutation are picked up from the pregenerated two 'repositories' of the random binary digits.
Each mutation was picked at random from many possible alternatives (see 'Phenotypes accessible through mutation' below).
This mutation was picked for our analysis because it has been shown that it significantly lowers gene and protein expression levels (Pan et al., 2008).
The system provided a mutation list that showed the mutations that were picked up by the mass spectrometer.
Individual ESC clones were picked and sequenced to identify mutations.
Ten plaques with relative larger size were picked to further identify potential mutation in the siRNA targeting region by sequencing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com