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Mutations were performed in a series of steps.
The DAAM1 N-terminal point mutations were performed using a two-step PCR method.
Mutations were performed with the QuikChange Site-directed Mutagenesis Kit (Stratagene, USA).
Mutations were performed using a QuikChange™ site-directed mutagenesis kit (Stratagene).
Ser27Ala, Ser47Ala and Thr530Ala mutations were performed by using site-directed mutagenesis.
For each of the 100 snapshot structures of the MD simulation, the following in silico mutations were performed.
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Screening for BRCA1/BRCA2 mutations was performed using denaturing high performance liquid chromatography (dHPLC).
The fraction of the initial population is selected with a probability and then mutations are performed on them.
Most testing for JAK2 mutations is performed by analyzing the genomic DNA of the JAK2 gene.
Structural analysis for all mutations was performed using Coot [ 36].
Analysis of mutations was performed as previously described.
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transfer were performed
deployment were performed
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mutations were captured
mutations were selected
mutations were listed
mutations were described
mutations were detected
mutations were eliminated
mutations were analyzed
mutations were determined
mutations were confirmed
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