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The point mutations were obtained using an overlapping PCR strategy described in detail elsewhere [64].
dFKBP59 and Atox1 mutations were obtained from the Bloomington Drosophila Stock center (http://flystocks.bio.indiana.edu).edu
Mutations were obtained by the QuickChange Site-Directed Mutagenesis Kit (Stratagene).
There is also the possibility that these mutations were obtained as an adaptation in chicken eggs.
Briefly, point mutations were obtained by PCR using forward and reverse overlapping mutagenic primers (Figure S3 and Table S2).
DRIL1 SUMO mutations were obtained by site-directed mutagenesis using the Quick-Change Site Directed Mutagenesis kit (Stratagene).
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If one factors in the presence of mutator mutations, or other mechanisms of genetic instability, a proportionately higher number of mutations is obtained.
According to preliminary results definite diagnosis based on disease-causing mutations was obtained directly in 15 patients and probable disease-causing mutations were found in another 12 patients.
Genotypic confirmation of all mutations was obtained by PCR using primers flanking the deleted region (data not shown).
The information on cancer-related gene mutations was obtained by screening the database Cosmic [ 37].
Complementation of the mutations was obtained by introduction of the low copy plasmid pMR20 [ 47] carrying either znuK or znuL.
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separations were obtained
developments were obtained
times were obtained
transformants were obtained
transfer were obtained
mutations were gain
mutant were obtained
mutations were found
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mutations were selected
mutations were described
mutations were detected
mutations were eliminated
mutations were verified
mutations were confirmed
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