Sentence examples for mutations were mixed from inspiring English sources
When the introduced mutations were mixed at 1%, five of the six had z-scores greater than any unexpected mutation.
Three FFPE tumour DNA samples known to harbour BRCA pathogenic mutations were mixed with a single background non-mutant FFPE tumour DNA to make 1/2, 1/4, 1/8 and 1/16 admixtures.
Our conclusion that each Ire1 monomer contains a complete catalytic center in the context of the Ire1 oligomer agrees with the model proposed previously based on experiments in which several Ire1 variants with dissimilar RNase-inactivating mutations were mixed [ 6].
Briefly, pEGFP-N3-SDC-4 (50 ng) and primers (125 ng of upper primer and 125 ng of lower primer) for each mutation were mixed with reaction buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.5), 1 µL dNTPmix and 2.5 U PfuUltra HF DNA polymerase, and PCR was performed (1 step 30 seconds at 95°C, 18 stages of 3 steps (30 seconds at 95°C, 1 minute at 55°C, 6 minutes at 68°C).
For each mutation, the purified extracts were mixed with the pMD19-T simple vector master mix (Takara BIO) according to the manufacturer's instruction and ligated at 16°C for 2 hours.
Students' attitudes were mixed.
However, findings were mixed.
Conclusions from previous studies on the SMA patient mutation D44V have been mixed.
The minimum read percentage with non-reference nucleotide is set universally for each gene and each library once the quality cut-off is set, while the sequence depth for each gene and library may vary so that for a certain genes or libraries the true mutation calling may be mixed with noise.
