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Multiple regions from a single tumour region were subjected to sequencing and mutations were mapped onto a phylogenic tree to illustrate the evolution of mutational events.
All mutations were mapped onto the available crystal structures for Arf1p: Arf1p bound to GDP, to GTP, and complexed with the regulatory proteins ArfGEF and ArfGAP.
Most of these mutations were mapped with traditional methods, using both visible markers and individually amplified Single Nucleotide Polymorphisms (SNPs) to score meiotic recombination events.
Co-occurring mutated genes were first mapped into 183 human signaling pathways, and 42 pairs of genes with co-occurring mutations were mapped to the same pathways.
The affected loci were then identified using both complementation tests with previously identified alleles, and DNA sequencing of candidate genes in regions to which the mutations were mapped.
Based on a more accurately resolved structure, Fleishman et al. [11] proposed a model for the arrangement of Cα carbon atoms in the TM helices which, owing to the wealth of data from patients with naturally occurring CMTX mutations, were mapped onto the amino acid sequence of Cx32.
Similar(26)
More than 400 mutations are mapped.
Fixed mutations are mapped onto the trunk of the tree, lost mutations onto off-trunk branches.
This information is available for each gene and all mutations are mapped to these standard sequences.
47– 51 These mutations are mapped on the Tm sequences shown in Figure 12.
For homologous group 1 and 2, more number of mutations was mapped on short arm whereas for chromosome group 4, 5, and 7, more number of mutations was mapped on long arm.
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