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The males from G2 were then coupled with Kisumu females so that at the third generation only the progeny of couples of which the male was free of target-site mutations were maintained.
Most mutations were maintained into the chronic phase of HCV infection (75%).
Generation and genotyping of the Dlx5LacZ/+, Dlx5/Dlx6neo/+, Krox20LacZ/+, Et1neo/+ have been previously described [37], [39], [51], [69], these mutations were maintained on the B6D2F1 hybrid genetic background.
These mutations represent the background mutations in which the lethal mutations were maintained.
All lethal mutations were maintained over balancer chromosomes containing either a lacZ or GFP transgene to allow for unambiguous identification of homozygous mutant embryos.
To facilitate the identification of homozygous mutant embryos, lethal Pex1 mutations were maintained over the w –; Sb /TM3, P{ActGFP}JMR2, Ser balancer.
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Most of these mutations are maintained into the later phase of infection.
Furthermore, a reference list of resistance mutations is maintained by the International AIDS Society IASS) [3].
In the presence of purifying selection, an excess of mutations in external branches is likely to occur as deleterious mutations are maintained at low frequencies.
These mutations are maintained as heterozygous mutations using translocations and duplications.
The summary of EOFAD mutations is maintained at the Alzheimer Disease and Frontotemporal Dementia Mutation Database [ 17, 18].
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mutations were hitherto
mutations were found
mutations were captured
mutations were selected
mutations were listed
mutations were described
mutations were detected
mutations were eliminated
mutations were confirmed
mutations were introduced
mutations were identified
mutations were analyzed
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