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Mutations were introduced into the plasmid pET28a containing the RrQR gene.
The point mutations were introduced by PCR using the oligonucleotides described in Table 1.
Mutations were introduced to the domain-swapped homodimer of the antiviral lectin griffithsin (GRFT).
Null mutations were introduced to bring down these ΔG levels below −5.0kcal/moll.
To make sure no mutations were introduced during above PCR cloning, the newly constructed plasmids were sequenced.
Mutations were introduced by two-step PCR-based targeted mutagenesis.
Mutations were introduced using the QuickChange site-directed mutagenesis kit (Stratagene) as per the manufacturer's recommendations.
Mutations were introduced by PCR-mediated site-directed mutagenesis using the Quikchange kit (Stratagene).
Mutations were introduced into the ICP0 gene of this plasmid, as described below.
Human preproinsulin cDNA was subcloned into pTarget (Promega) and the mutations were introduced as described above.
Point mutations were introduced by site-directed mutagenesis using the Quikchange™ kit (Stratagene).
More suggestions(15)
transfers were introduced
mutations were inserted
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mutations were hitherto
mutations were found
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mutations were selected
mutations were described
mutations were detected
mutations were analyzed
mutations were verified
mutations were eliminated
mutations were observed
mutations were confirmed
mutations were made
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