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In the double mutants and a triple mutant, the single mutations were incorporated in various combinations.
The mutations were incorporated into wild-type full-length Drosophila Notch (described in [18]) in both the pMT [19] and pUAST [20] vectors.
The importance of the EBox as the primary mediator of the AICAR effect on PGC-1α promoter activity was further evaluated by generating a reporter construct in which mutations were incorporated into the Ebox sequence of the overlapping GATA/EBox sequence within the p851 promoter length.
The resulting pET16b-PAD2 construct was sequenced to ensure that no mutations were incorporated.
We verified whether the desired mutations were incorporated by sequencing the TEM locus.
All clones were sequenced to confirm that only the intended mutations were incorporated into the constructs.
Similar(44)
The mutations are incorporated into the models by substituting the amino acids during structure modeling.
Moreover, we describe two mutations in antigenic sites that may cause antigenic drift in the virus as more mutations are incorporated in these sites.
FUS/TLS with ALS-causing mutations is incorporated into cytoplasmic stress granules under stress conditions and is important in the pathogenesis of ALS [ 24– 27].
This states that sex functions solely to increase the efficacy with which beneficial mutations are incorporated into populations, but that sex has no bearing on the efficacy with which selection operates on detrimental mutations.
In other words, the T22M mutation leads to selective depletion of the isoform 1, the only isoform in which this mutation is incorporated.
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CEO of Professional Science Editing for Scientists @ prosciediting.com