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Somatic mutations were further investigated in another 14 patients with advanced SM.
Double and triple mutations were further conducted and four mutants H275/293D, H275/310D, H293/310D, and H275/293/310D were obtained.
Plasmids containing the correct mutations were further verified by sequencing.
Transcriptional sequences (cDNA sequences, GQ354123 8) of three accessions with the D7 and D10 mutations were further determined, respectively.
Mutations were further filtered by excluding events which were classified as "somatic," "synonymous" or "silent," or "unvalidated," A list of the sample IDs and the summary of genomic data are given in Table S3.
Nonsynonymous mutations were further distinguished as missense or truncating.
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The pathogenicity of the mutations was further corroborated by the high conservation of the mutated amino acids.
When these mutations are further combined with mutations at S416 and S419, the turnover of BHET can be increased 120-fold compared to wild type (Fig. 4c).
Mutations in Pol β have been linked to various cancers, and these mutations are further correlated with altered Pol β enzymatic activity.
The complexity involved in analysis of activating mutations is further demonstrated by the difficulty in categorizing mutations found in protein databases as deleterious, neutral, or activating.
The stability of the introduced mutations was further checked in viruses recovered from latently infected spleens confirming the retention of the engineered point mutations.
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