Sentence examples for mutations were engineered from inspiring English sources

Mutations were engineered in E, NS3 and NS4B protein genes to improve replication in Vero cells.

The mutations were engineered both at the donor (L131L, M160L, M197F and M202H) and acceptor (M265I and M234E) sides.

HCM-associated JPH2 mutations were engineered and functionally characterized using immunocytochemistry, cell morphometry measurements, and live cell confocal calcium imaging.

To assess their effect on antiviral activity of Oas1b, the NS3 and 2K mutations were engineered into an infectious WNV cDNA clone.

Mutations were engineered into the VLP genome to produce particles deficient in activities associated with viral reverse transcriptase, RNase H, and RNA packaging.

Mutations were engineered by Quick-change mutagenesis (Agilent Technologies) in a pcDNA3.1-Kv7.2 pcDNA3.1-Kv7.2lectroplasmidogical and western-blot experiments), or in a dual-tagged Enhanced Green fororelectrophysiological-hemandlutinin (EGFP-Kv7.2-HA) plasmid (for immunocytochemistry experiments), as described21,34,61.

These mutations are engineered into a recombinant genome that is inserted into a cell, where it generates a recombinant virus.

The panel of amino acid mutations was engineered by PCR-based mutagenesis from the cDNA encoding wild-type proteins using Pfu DNA polymerase (Promega, M774B) and primer sets depicted in Table S2.

The linker region and nearby residues where site-mutations were engineered into E. coli CtpS is boxed in black.

A single point mutation was engineered within Annexin A2-expressing vector, with resulting vectors called pEXS-Annexin A2-K302A and pEXS-CG-Annexin A2-K302A.

The argT (STM 2355) mutation was engineered in serovar Typhimurium following the one-step deletion strategy as described by Datsenko [35].