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The crystal structures of M. jannaschii tyrosyl-tRNA synthetase and its mutations were determined, which provided an opportunity to design aminoacyl-tRNA synthetases specific for other unnatural amino acids.
By analyzing the targeting site on the genome of corresponding transgenic plants, the mutations were determined.
Gag mutations were determined in relation to the estimated time of seroconversion.
The prevalence and profile of drug-resistant mutations were determined using Sanger sequencing and ultra-deep pyrosequencing.
Mutations were determined by direct sequencing.
The C357T and G428A mutations were determined by PCR-RFLP.
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The final catalog of mutations is determined by the strength and duration of exposure to each mutational process.
However, as new resistance-causing mutations are determined, Mykrobe predictor can be easily updated and tested, and unlike a line-probe assay or the automated Xpert-Mtb/Rif (Cepheid) assay, is unaffected by number of resistance-causing mutations in the panel.
During three generations of replication, possible mutations are determined by the outcome of roulette spins.
The identity of the mutations was determined by sequencing.
The purified clonal fragments were then sequenced (20 50 clones per sample) and linkage between integrase mutations was determined.
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mutations were described
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mutations were confirmed
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