Sentence examples for mutations were designed from inspiring English sources
Fourteen potentially stabilizing mutations were designed and introduced into scFvD1.3 by site-directed mutagenesis, either individually or in combinations.
Seven different amino acid mutations were designed and introduced to the position 461 of the 1a protein in RNA 1.
The mutations were designed to increase the helical propensity of the regions of the polypeptide chain corresponding to the two α-helices of the native protein.
Mutations were designed based on the available structural data of SifA and its interface with SKIP, or modeled for SifA as putative guanine nucleotide exchange factor.
To investigate residues that are critical in directing the reaction pathway, seven point mutations were designed based on the differences between Asd and amiontransferases in conservative amino acid residues.
Two steps were followed to study the activation mechanism of CheY upon phosphorylation: first, we independently substituted the three aspartic acid residues in the active site with alanine; second, several mutations were designed in helix α4, both to increase its level of stability and to improve its packing against the protein core.
The one-point crossover and repairing strategy for mutations are designed to avoid infeasible solutions.
Typical mutations are designed to disrupt or map protein protein interactions, mimic or block posttranslational modifications, or to silence enzymatic activity.
This idea should not be surprising, because traditional promoter analysis (5' deletions, gain-of-function-experiments by core promoter swaps and point mutations) is designed to identify at least one functional elementfor the expected biological response, and not to determine the entire promoter structure.
Primers used for mutation were designed using the QuikChange Primer Design Program (http://www.stratagene.com/sdmdesigner/default.aspx).aspx
For simplifying the process of cell disruption and immobilization, the lysis genes of phage λ (S−RRz) with the S amber mutation were designed to introduce into the over-expression system of GLA.
