The DNA samples have a low frequency of mutations, and these mutations are composed almost entirely of single-nucleotide codon changes these samples quantify the baseline error rate from PCR and deep sequencing.
The other haplotypes in the first group, which did not carry mutations in the coding sequence, were composed of a mixture of aromatic and non-aromatic accessions.
We reasoned that if the intranuclear protein crystals formed at the late stage of Ad5 infection were composed of pentons, certain mutations in one or the other domain of the protein, penton base, fiber shaft or fiber knob, might affect the occurrence and/or structure of the crystals and hopefully result in structural alterations visible under the EM.
Cloning-sequencing confirmed that ∼99% of the TaqI-RE-digestion-resistant RT-PCR clones from PBMCs were composed of the R46X mutation (Table 1).
The negative subsets were composed of COSMIC or WG somatic mutations occurring in other genes and are not recurrent in the corresponding dataset (Table 2).
Our series were composed of 16 cases of breast carcinoma in women with a germline Brca1 gene mutation, and of four cases with Brca2 mutation.
They were composed of three sections.
To generate mutant templates to use as positive controls, a mutation-specific primer is composed of 45-mers of mutant sequence with 10 15 nucleotides complementary to wild-type sequence at the 3' termini for annealing to the wild-type template [16].
As the mutant phenotype is composed of all the mutant sequences except the master sequence, back mutation can be neglected as a first approximation.
Note that a mutation in the current model can be composed of multiple substitutions (non-adjacent mutation) because the average number of substitutions per replication, (1 - q) ν (commonly denoted by U in the population genetics theory), can be greater than 1.
In other words, 20%% of the succeeding generation is composed of mutation and inversion operations.
