Sentence examples for mutations were calculated from inspiring English sources

Potential disease-associated point mutations were calculated using the Site Directed Mutator (SDM, http://mordred.bioc.cam.ac.uk/~sdm/sdm.php).ac.uk/~sdm/sdm.php

Next, the numbers of C→T and G→A changes from the wild type sequence were counted for each position per pool (Fig. 3) and the probabilities that they represent true EMS mutations were calculated taking into account their distribution across the 3D sample pools.

Rates of non-synonymous (Dn) and synonymous (Ds) mutations were calculated using DnaSP 4.50.3 software available at http://www.ub.es/dnasp/[41] in order to determine the Dn/Ds ratio for the ibeA locus and concatenated MLST sequence data sets of E. coli strains allocated to STC95 (n = 21).

Similarly, P SH ≤ SH_r) values for the sets of reconstructed synonymous mutations, common SNPs and de novo mutations were calculated.

Correlation within strains to synonymous, nonsynonymous and noncoding mutations were calculated from Spearman's non-parametric correlation coefficient.

Frequencies of gpt gene mutations were calculated by the number of 6-TGrCmr colonies harboring independent mutations divided by the number of Cmr colonies.

Concurrent reversion rate between two given mutations was calculated as the rate of simultaneous reversion of these 2 mutations between 2 consecutive timepoints (Equation 1).

The frequency of mutations was calculated.

The expected number of alleles that were a result of point mutations was calculated.

The surviving fractions for genomes with different numbers of mutations are calculated as follows.

The Fst value for all non-synonymous mutations was calculated using Genepop 4.2 [ 21].