Sentence examples for mutations were assayed from inspiring English sources

When these mutations were assayed in the backbone of an EGFP-SMNex1-5 thencation, the D44V mutation no longer blocked calpain cleavage, further supporting a role for the C-terminus in regulating availability of the cleavage site (Fig. S6).

Only a subset of the mutations were assayed.

In consequence, primary mutations were assayed but secondary mutations were not included.

PDR 1 and PDR3 gene deletion mutations were assayed to confirm their influence on the expression of the potential regulons.

The identified mutations were assayed in 460 control chromosomes from 230 healthy Japanese individuals by direct sequencing.

DOI: http://dx.doi.org/10.7554/eLife.07918.015 To further explore the in vivo relevance of these findings in the context of long-term differentiation as well as tumor progression, cells with TERT promoter mutations were assayed for teratoma tumor formation in immune-compromised mice.

The hERG-FinB mutation was assayed with restriction enzyme analysis (Fodstad et al., 2006) by amplifying the genomic DNA with primers for hERG (forward: 5′-ACCACGTGCCTCTCCTCTC-3′, reverse: 5′-GTCGGGGTTGAGGCTGTG-3′) (reagents from Applied Biosystems) and digesting the amplified PCR product with SmaI digestion enzyme (Fermentas GmbH, St Leon-Rot, Leon-Rot, Germany

Point mutation frequencies were assayed using the transgenic A. thaliana (accession Columbia) lines 693, 699, 747 and M4.

A total of 768 mutant lines were assayed for mutation induction in the target genes.

Wild-type (WT) IN and IN with mutations Y143C or Y143R were assayed in vitro in 3'end-processing, strand transfer and concerted integration assays.

The DTCs were cultured for 21 days and single cells were assayed for mutation detection.