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To more carefully control for the effects of second-site mutations, we replicated the reciprocal hemizygosity test using deletion strains that were backcrossed to their parents.
To determine if the bubble region influenced the types of mutations, we replicated a construct in which C8-dG-ABA was placed in a similar bubble.
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Mutations are replicated when the cell replicates.
This review summarizes genes harbouring causative rare mutations and replicated common predisposing alleles.
Importantly, the IKBKAP association with HSCR bearing RET CDS mutations was replicated in the same Chinese population.
The mutations in LesBMUT1 replicated the electrostatic congruence in the MUT1 residues, but not in the MUT2 residues.
Klenow polymerase has been shown to have an average error rate (mutations per base replicated) of 1.3 × 10−4 [ 18].
For all samples with possible mutations, sequencing was replicated in forward and reverse orientation using an additional affected family member (or using the original patient if no other affected individual had been sampled) in order to confirm the presence of the mutation.
One particular chimeric virus, bearing E protein mutation Q264H, replicated to higher titer in tissue culture and was significantly more immunogenic in mice.
In each generation, all DOs will undergo random mutations and replicate, resulting in 200 DOs.
To evaluate reproducibility, tumour samples that were found to contain a significant mutation were replicated in at least duplicate.
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