Sentence examples for mutations we knocked from inspiring English sources

By using the CRISPR/Cas9 genomic editing tool to generate the insertion or deletion mutations, we knocked out the OsLAP6/OsPKS1 gene and obtained many its loss-of-function mutants.

To determine the myosin knockdown phenotype and compare it with that of hsp90α1 mutation, we knocked down smyhc1 expression using a smyhc1-specific translational morpholino (ATG-MO) in zebrafish embryos (Fig. 1).

This possibility was supported by an in silico mutation analysis, in which we knocked out the positive feedback circuit (processes 21 25 and the related elements) in the simulation model.

20 We knocked-in this mutation into the genome of C57 ES cells in order to generate syngenic C57 mice without requiring extensive backcrossing needed to obtain homogenous C57 genetic background if 129 ES cells were used.

To investigate whether these transgenic flies can be used to efficiently produce site-specific mutations, we set out to knock-out ms(3 k81 using a previously designed gRNA (Yu et al. 2013).

To gain insight into how specific Met mutations induce tumorigenesis, we utilized the panel of knock-in mutations we previously generated.

Researchers have suspected for about a decade that Foxa2 turns on surfactant genes, but they couldn't test this in animals because any mutations that knocked out Foxa2 killed the embryos too early in development.

First, mutations that knocked out gene 1.5 appeared in T7 and both lines of T3.

Accordingly, PKD2 S707andnd S711A mutations were knocked into the wild-type Prkd2 locus in mouse ES cells by homologous recombination.

We designed Doc mutations to knock out each binding mode, and compared their single-molecule unfolding patterns as they were dissociated from Coh using an atomic force microscope (AFM) cantilever.

To understand the role of Met in tumor development and progression, we have utilized a unique mouse model that allows us to compare four different mutations knocked into the Met genomic locus [19].