Sentence examples for mutations we established from inspiring English sources

For the simultaneous detection of the predominant K-ras codons 12 and 13 mutations, we established a sequencing protocol based on the design of a single PCR primer pair and a single sequencing primer.

Using HMW1ct derivatives with double mutations, we established that ApHMW1C can transfer the glucose moiety from UDP-glucose to all three of the documented glycosylation Asn sites within HMW1ct but can transfer the galactose moiety from UDP-galactose to only two of the three Asn sites.

To probe the functional significance of the GNAS mutation, we established a colorectal cancer cell line stably expressing GNASR201H.

To determine whether FMR1 mRNA and protein levels were affected by the G-insertion mutation, we established an immortalized EBV lymphocyte line from a blood sample of the patient and measured FMR1 mRNA and protein levels in these cells.

As another component of the new mutation assay, we established a custom-designed test allele that contains a microsatellite sequence as a target for mutations.

After the identification of the mutation in 2010, we established a direct test to detect carriers among artificial insemination sires.

To determine the relative contribution of the PTEN ATP-binding mutations in breast carcinogenesis, we established clones with stable PTEN expression controlled by a Tet-off system to examine the consequences of increased levels of wild-type (WT) and mutant PTEN expression in a well-characterized breast cancer line, MCF-7, as we described before (9, 11).

To assess the important role of USP8 mutation in ACTH production, we established surgically resected primary human ACTH-secreting tumor cells.

We established that mutations of T426 alter the KaiC phosphorylation profiles in vivo and that residue 426 needs to be phosphorylatable and not simply capable of forming a H-bond to pS431 [28].

As described in McGuigan et al. (2011), we established 100 mutation accumulation (MA) lines from a laboratory-adapted population of D. serrata, which had previously been subjected to 13 generations of full-sib inbreeding.

To determine the effects of the mutations, we used an established functional assay in which laminin-111 polymerization is inhibited by soluble short-arm fragments (Yurchenco & Cheng, 1993; Cheng et al, 1997; Garbe et al, 2002).