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To examine the potential genomic linkage of reversion of pairs of mutations, we calculated the frequency of pairs of RT mutations reverting concurrently between baseline and 2 months of STI.
In order to investigate SNAP's ability to distinguish activating and neutral mutations, we calculated the percentage of mutations called neutral for each of the three categories at each reliability index (Figure S3).
DOI: http://dx.doi.org/10.7554/eLife.11282.007 To measure the rate at which the virus population accumulates mutations, we calculated the average distance of each sample from the approximate founder sequence in 300 bp windows.
For the pseudogene set with accumulated mutations, we calculated a median dN/ dS of 0.50 indicating a considerable relaxation of selective constraints of the canine pseudogenes in comparison to their human functional orthologous.
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First, with the simplification of neglecting back mutations, we calculate the error-threshold as the maximum allowable mutation rate for which the fittest genotype can survive.
To further investigate possible role of mutation, we calculated the GC content of noncoding sites of the 7 genes (Additional file 4).
Because a genotype on the line of descent necessarily differs from its parent by at least one mutation, we calculated the probability that a particular genotype would differ from its parent genotype by two or more mutations.
Because student models had a variable number of propositions containing mutation, we calculated mean accuracy scores for each model as the sum of all points assigned to mutation-containing propositions divided by the number of propositions (e.g., Student A used mutation in two propositions, rated 2 and 3 points; her mean score was (2 + 3)/2 = 2.5).
Thus, in this example to have a > 90% chance of identifying at least one isolate containing only the targeted mutation, we calculate that 0.90 < 1-S xx, or x = log (1-S xlor (0.90).
To estimate the percentage of the genome modified in individual animals or larval samples (i.e., mutation rate), we calculated the percentage of mutant PCR products (uncut, or short deletion bands) relative to the total PCR product.
Using 1000 randomly selected simulations per mutation rate, we calculated the mean number of observed genotypes per sub-population for each mutation rate.
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