Sentence examples for mutations was then from inspiring English sources

A full length product containing the desired mutations was then PCR amplified using JK6, JK7, and the products of the first two reactions as template.

The epistatic interaction E of the mutations was then calculated according to the epistasis definition in a two-locus-two-allele model: E = W00W11−W01W10 [Equation 1], where W00 is the fitness of the wild type, W11 the fitness of the double mutant and W01, W10 are the fitness of both single mutants, respectively.

Analysis of phenotypes arising from the mutations was then performed in vivo.

The total number of BRCA1 mutations was then obtained by summing π j 1 over all families.

Analysis of DMD genetic mutations was then carried out for the 7,149 patient data sets held within the TREAT-NMD DMD Global database.

The net change in the number of deleterious mutations was then calculated and the virus's mutation load was appropriately increased or decreased.

These mutations are then perpetuated.

The mutations were then arranged in a family tree.

Combinations of three point mutations were then created via site-directed mutagenesis.

These mutations were then prioritized by other methods of design, mainly the formation of additional hydrogen bonds, an increase in the hydrophilicity of solvent exposed residues, and previously described mutations in other antibodies.

Additional mutations were then introduced by P1-mediated transduction and selection for the appropriate antibiotic resistance.