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The perturbation in stability induced by the mutations was quantified
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Mutations were quantified using the machine's software.
After PCR amplification of a panel of previously established murine microsatellite markers, frameshift mutations were quantified as NMPM in comparison with tail DNA (figure 4A).
Perturbations in amide resonances resulting from the mutation were quantified using chemical shift perturbations (CSPs) calculated as CSP = [(DdH 2+(DdN/5)2]1/2, where DdH and DdN are the observed chemical shift changes for 1H and 15N, respectively.
The chemical shift changes due to this mutation are quantified using eq 3 and shown in Figure 2B for each residue.
For the m.1555A→G mutation, heteroplasmy was quantified by cloning and sequencing of 23 independent mtDNA fragments from an independent aliquot from the original DNA sample.
The complexity of mutant spectra was quantified by minimum and maximum mutation frequencies.
For validation, an independent cohort of 150 normal karyotype AML was investigated for RUNX1 mutations (Table 1b), CEBPA expression was quantified using real-time RT-PCR.
In order to further test the hypothesis that a slight impairment on PrPC cleavage could be mildly toxic and therefore be translated into human diseases, the degree of proteolysis of constructs carrying mutations linked to human prion diseases was quantified.
Effect of Atg6 null mutations on Notch EC domain localization was quantified by selecting 30 × 30 pixels measurement areas containing clone or control cells exclusively.
Microgliosis was quantified similarly.
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