Sentence examples for mutations was prepared from inspiring English sources

By comparing the sets from 12 M2' plants to each other, a variant filter to distinguish unrelated variation from mutations was prepared based on a common frequency threshold of 20%%.

The SH3 domain mutations were prepared using the Quikchange protocol (Statagene) and verified by DNA sequencing.

Recombinant infectious clones of HIV-1 carrying various mutations were prepared using standard site-directed mutagenesis protocols as described previously [38].

All mutations were prepared by PCR-based mutagenesis of the 0.21 kb promoter region in pcDNA3.1Luc plasmid, using the QuickChange II XL Site-directed mutagenesis kit (Stratagene) according to manufacturer's procedures and oligonucleotide primers as in supplementary Table 1.

To test the functional relevance of the candidate transcription factor binding sites, point mutations were prepared to inactivate each of the identified conserved motifs in the context of the −219/+114 FSCN1 luciferase reporter plasmid (Fig. 4A, Fig. 5A).

ErbB2 exon 20 insertion mutations were prepared using the method described above.

As the likelihood of having a significant representation of rare IDH1/2 mutations in the clinical cohort was low, synthetic samples representative of all 11 rare mutations were prepared.

Site-specific point mutations were prepared with the QuikChange site-directed mutagenesis kit (Stratagene) and expressed and purified following the same protocol as described for wild-type crammer.

Synthetic RNAs corresponding to specific fusion transcripts, splicing variants or mutations were prepared using standard in vitro transcription methods and diluted in HL60 total RNA keeping the concentration of total RNA constant.

To estimate the sensitivity of the applied techniques, serial dilutions of DNA from NCI-H1650 NCI-H1975975 lung cancer cell lines containing delE747-A750 and L858R mutations were prepared using DNA isolated from healthy donor peripheral blood mononuclear cells (100%% wt and 50, 25, 20, 10, 5, 2 and 1 % mutant DNA by volume).

DNA from a mutant strain carrying one of these mutations (e3016 ) was prepared for whole genome sequencing using a standard library preparation without amplification, followed by Illumina 50-bp paired end sequencing on the HiSeq2000.