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A panel of mutations was introduced into residues substantially displaced upon the G93A mutation.
In addition, off-target event analysis revealed that none of the off-target mutations was introduced into the embryos.
To identify the sequences and structural elements within the 5' NCR of HCV RNA that contribute to the initiation of translation, a series of point mutations was introduced within this sequence.
Thus it appears that HHT in the Netherlands Antilles is due to a limited number of ancestral mutations in the endoglin gene, and that one of these mutations was introduced into the African slave population by a Dutch colonist.
containing the E2 60D, 162A, and 211T mutations, was introduced into 41855-GFP-226V (supplemental data Fig. S1).
Plasmid vector pTJA-57 (pKSV7::ΔsigB, kind gift of Dr. Kathryn Boor) [84], a pKSV7 derivative designed for the construction of ΔsigB mutations, was introduced into NF-L943 (prfA G155S) by electroporation as previously described [86].
Similar(51)
To make double mutants, the U110 mutations were introduced into the A206 mutated templates.
In addition, five thermostabilizing mutations were introduced.
K519A or K833A mutations were introduced into the cloned OsIRE1.
Mutations were introduced into the KGSADH gene, using the assembly PCR method.
Single specific mutations were introduced into nucSTB by PCR with the suitable mutagenic pairs of primers.
More suggestions(15)
mutations was combined
mutations was verified
mutations was obtained
mutations was based
mutations was led
mutations was confirmed
mutations was constructed
mutations was coupled
mutations was determined
mutations was followed
mutations was carried
mutations was observed
mutations was simulated
mutations were introduced
mutations was identified
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