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Recently a more relevant mouse model was created in which humanized Aβ, with human AD-causing mutations, was inserted into endogenous mouse APP [ 144].
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The mutated gene was sequenced to confirm that only the desired mutations were inserted.
Matthew B. Frieman, a virologist at the University of Maryland School of Medicine, said that a review of the scientific literature could point to where the mutations were inserted.
The aforesaid mutations were inserted into the strain by using pYX212 vector.
Site-specific mutations were inserted in primer sequences in order to prevent extension of reciprocally complementary primers.
Three pairs of mutations were inserted.
Out-of-frame ATG (uATG) mutations were inserted at −6 bp into the WT, the DPE-L1/2 double mutant and the GLE mutant, transiently transfected into HeLa cells and assayed for CAT activity.
Mutations were inserted by recombinant PCR as described previously [29].
Site specific mutations were inserted with COOT [ 24].
All point mutations were inserted by site-directed mutagenesis.
Mutations were inserted with specific primers via site-directed mutagenesis by overlap extension.
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