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To determine whether a change in binding or peptide responsiveness with the mutations was due to perturbed cell surface expression, we performed whole-cell elisas.
To ensure that the lethality of the newly generated mutations was due to mutation of Top2, we tested whether the P[Top2-w +] transgene that carried a wild-type Top2 gene rescued the viability of Top2 m/Top2 Dflieslies.
In contrast, mutations in COL1A1 that alter residues in triple helix regions 328 346 and 418 436, proposed to be of critical importance due to a lack of observed mutations (5), contain four mutations in our data set (Gly331Val, Gly424Arg, Gly427Arg and Gly436Arg), suggesting that the previous absence of mutations was due to sampling.
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On average, 66.50% mutations were due to INDELs with varying degrees among the four strains.
A cancer is 'nongenetic' (sporadic) in origin when the set of cancer-causing mutations are due to somatic mutation alone.
The clinical dilemma of managing pregnant women with GCK mutations is due to uncertainty about the fetal genotype.
In order to know if the mutations were due to changes in repeat copy number or to point mutation events, the mutational products and parental alleles were sequenced.
If both double and complex mutations were due to error-prone double-strand break repair, then the observed number of these mutations accounted for ∼1% of SNMs.
We estimate that ∼50% (and at least 37%) of shared derived mutations are due to incomplete lineage sorting rather than recurrent mutation.
This vast scattering of TP53 mutations is due to the marked fragility of the core domain, which can be destabilized by amino acid substitutions at many different positions.
The greater severity of PKD1 mutations is due to the development of more cysts at an early age, not to faster cyst growth [ 2].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com