Thus, the probability of transformants having no untargeted mutations is considerably increased both by using high-fidelity Taq polymerases and by minimizing the size of the PCR fragment.
The frequencies of the c. 1080C>T and c.*543T>C variants were substantially higher in the Czech population whereas the frequency of the c. [833C; 844_845ins68] double mutation was considerably lower than in Africans.
We show that the number of abasic sites is significantly increased when the 3-methyladenine DNA glycosylase (Mag): AP endonuclease (Apn1) ratio is increased and that spontaneous frame shift mutation is considerably elevated when either Mag, or Mag plus Apn1, expression is elevated.
The assay detected ≥ 10% mutated mtDNA in each case, and for some mutations, the detection threshold was considerably lower (Table S1).
However, transmission of TALEN-induced mutations to the next generation was considerably lower at the ADH1 locus (69% for ZFNs vs. 12% for TALENs).
The frequency of typical mutations, such as albino phenotype, was considerably higher in our library than in previously reported soybean mutant populations [ 40, 41].
However, the actual number of mutations between these two haplotypes was considerably less at 23 (equating to 4.7% divergence) suggesting that there are a number missing links within the network and that it is poorly resolved.
For the groups with a reasonable number of mutations, the performance of the classifier was considerably better than with the less populated groups.
Various substituents on the aromatic ring system are freely tolerated: Although wild-type galactose oxidase from Fusarium has a broad substrate scope for benzylic prim-alcohols, the activity was considerably increased by mutations.
