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Direct testing for p53 LOF mutations was carried out by DNA sequencing.
Site-directed mutagenesis of PaHNL1 to introduce His459Asn and His497Asn mutations was carried out using the Stratagene QuickChange XL kit.
Analysis and interpretation of polymorphisms and other drug-resistance mutations was carried out using the Stanford HIV drug-resistance database.
Genotyping of relatives of the probands found to carry PALB2 mutations was carried out via Sanger sequencing.
The estimation of DNA mutations was carried out using ion semiconductor sequencing on an Ion Torrent Personal Genome Machine (Ion PGM System).
Detection of MPL W515L/K mutations was carried out in a single PCR reaction with 2 outer primers designed to amplify the internal amplification control flanking the mutation site.
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The mitochondria are derived from the egg of the mother, and therefore these mutations are carried down the female line in perpetuity.
A female and male G1 offspring of two separate ENU-treated males were intercrossed to maximize the number of mutations being carried in the G2 offspring.
7 Protection of cells against accumulation of numerous mutations is carried out by DNA repair enzymes.
Mutations were carried out with the use of site-directed mutagenesis strategy.
Mutation calling and filtering was carried out using VarScan2 as described [ 6], annotation of coding mutations were carried out using ANNOVAR [ 7].
More suggestions(15)
variants was carried
developments was carried
move was carried
transfer was carried
mutant was carried
mutations was combined
mutations was obtained
mutations was confirmed
mutations was coupled
mutations was determined
mutations was followed
mutations was observed
mutations was introduced
mutations was simulated
mutations was identified
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