Sentence examples for mutations was amplified from inspiring English sources
Each fragment containing mutations was amplified by PCR and sequenced a second time to confirm that the identified mutations were not due to PCR artifacts.
The chromosomal DNA flanking regions used to construct the relevant suicide vectors for ΔPfur81 TT araC PBAD fur, ΔasdA33, and Δ tviABCDE10 mutations were amplified from the S. Typhi Ty2 chromosome.
The samples screened for BRCA1 and BRCA2 mutations were amplified using the BRCA MASTR kit v2.1.
Clones carrying the desired mutations were amplified and plasmids were extracted using NucleoBond Xtra Maxi EF (Macherey-Nagel, Bethlehem, PA).
The fragments containing the pre-defined mutations were amplified and sequenced using specific primers (Additional File 9).
Fragments of genomic DNA containing point mutations were amplified by PCR using the original purified fosmid as the template.
BRAF exon 15, which contains the V600E mutation, and KRAS codon 12/13 mutations were amplified using primers reported in Supplementary Table 1.
To exclude false-positive results leading to the large number of new mutations, direct sequencing analyses were performed in different laboratory conditions and the same mutations were amplified with concordant results proving that contamination was unlikely.
Full-length plasmids containing the designed mutations were amplified using the TransGen FastPfu DNA polymerase (TransGen Biotech) with pET-660 carrying the wild-type Sso0660 gene as the template.
Three regions of interest containing the previously identified mutations were amplified by PCR (Jebar et al, 2005) using the following primer pairs: for exon 7, 5′-AGTGGCGGTGGTGGTGAGGGAG-3′ and 5′-TGTGCGTCACTGTACACCTTGCAG-3′; for exon 10, 5′-CCTCAACGCCCATGTCTTT-3′ and 5′-GGGAGCCCAGGCCTTTCTTG-3′; and for exon 15, 5′-CCGCAATGTGCTGGTGAC-3′ and 5′-GGCGTCCTACTGGCATGA-3′.
The spx gene carrying the G52R mutation was amplified from chromosomal DNA isolated from ORB4055 using oligonucleotides oMMN01-173 and oMMN01-174.
