Mn2+ caused a dose-dependent increase in the accumulation of mutations using the CAN1 and lys2-10A mutassaysssassays
We established a new method for rapid screening of NPM1 mutations using the fluorescence resonance energy transfer (FRET) principle.
One of the most widely used omics technology platforms is mass spectrometry based detection of somatic gene mutations using the Sequenome DNA mass-array technology.
We studied the prediction of the energetic impact on PAH native-state stability of 318 PKU-associated missense mutations, using the protein-design algorithm FoldX.
We evaluated a new approach for rapid screening of Flt3-TKD and Flt3-V592A musingons using the fluorescence resonance energy transfer (FRET) principle in a group of 122 patients.
Compared with other published experimental methods for mutation analysis, the translation initiation can not be disturbed by multiple bases mutations using the proposed method, which shows the effectiveness of this method in improving the translation efficiency and its biological relevance for genetic regulatory system.
Table 1 also summarizes performance metrics (sensitivity, specificity, precision) for curated RET mutations using the four established prediction algorithms.
Sequences were assessed for nonsense mutations using the Gene Cutter program from the Los Alamos HIV Sequence database website (http://hiv-web.lanl.gov/content/sequence/gene-cutter/cutter.html).html
Tumor samples which were repeat positive for an aberrant melting curve were sequenced in duplicate, and the resulting sequence trace files were analyzed for mutations using the phrap/phred/consed software package (www.phrap.org).
Since 1997 all acid fast bacilli (AFB) positive samples in our hospital have routinely been sent to the Mycobacterium Reference Unit for testing for rpoB mutations using the INNO-LiPA Rif.TB assay (Immunogenetics, Zwijndrecht, Belgium) [9], [10].
We analysed Ki-ras mutations using the MASA method.
