Sentence examples for mutations to alanine from inspiring English sources
To test this hypothesis, mutations to alanine were made at positions I464, R466 and D469 as well as the double mutant I464A,R466A and the triple mutant I464A,R466A,D469A.
SSO1 genomic fragment (453 bp upstream of ATG and 501 bp downstream of stop) in B1473 was mutagenized using the QuickChange method (Stratagene) to generated S23, S24, S59, S79 mutations to alanine or glutamic acid.
While it is possible that some of these mutations affect the phosphorylation of the Cdc24 protein without changing its mobility shift, it appears that the mutations to alanine, especially of the PAK sites, perturbed Cdc24 phosphorylation.
Individual point mutations to alanine were introduced into the pep2 gene of M. smegmatis using the Quikchange approach (Stratagene, primers in Table S1, Supporting Information).
Mutations to alanine at these positions are known to disrupt cholesterol binding in human cells (Ko et al. 2003; Wang et al. 2010), raising interesting questions about the roles of these proteins in symbiotic cnidarians (see Discussion).
Mutations to alanine in the chelating residues strongly reduced the metal-induced stabilization observed in DSC; and, moreover, the double mutant E203A+D278A was sufficient to completely abolish binding to Zn2+ or Mn2+ in ITC experiments.
ASEdb gives the corresponding ΔΔG value representing the change in free energy of binding upon mutation to alanine for each experimentally mutated residue.
Deletion of the amino-terminal threonine or its mutation to alanine led to inactivation of the enzyme.
The effect on stability of mutation to alanine of all but two of the eight proline residues is correlated with their conservation within the family.
This invariant lysine residue in subdomain 2 coordinates ATP, and mutation to alanine results in significant inhibition of catalytic activity.
In most cases a mutation to alanine was done, but at three positions we also substituted these residues for Asp, Arg, or Pro (Table 3).
