Some mutations, though, have much higher frequencies than others, for example, p.Gly2019Ser and mutations altering codon Arg1441.
Line 7 specifies that each attempt involves only a single mutation, though again the structure of Stylus allows simultaneous mutations conforming to any frequency distribution.
A new neutral macronuclear mutation, though initially in the minority, can increase in frequency and eventually become fixed in a clonal lineage by assortment.
If different changes have occurred in the nucleotide sequence of the gene responsible for pale seeds in the different species, then lack of complementation in the F1 of a cross between two pale-seeded species does not prove identity of the underlying mutations even though the mutations are in the same gene.
This smoking parameter was only associated with cases with APC mutations, though this association was not statistically significant.
Most JEB cases also have enamel defects, including those due to recessive LAMB3 mutations, though these defects vary in severity.
Four mutations, though located in the introns, had a predicted effect on splicing and were classified as mutant (NNsplice 0.9, HSF_V2.3) (Petitjean et al., 2007).
Additionally, CAVA flagged that alternative annotations were possible for 34 mutations, though none altered the class (i.e., all possible representations result in a frameshift).
Of note, the dominant Sμ sequence in IgV-Mut #3 harbored five mutations even though the germ line Sμ sequence was still detectable within the subclones.
Likewise, up to 3.0 pg of injected Cas9 mRNA was not able to introduce efficient mutations even though the quantity of injected sgRNA is high.
