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However, for those mutations that prevented the rescue, it should be kept in mind that loss-of-function in vivo may also relate to other aspects.
Indeed, recently, has been proposed that ER-bound transcripts escape interactions with SGs during stress [14]; in yeast, mutations that prevented SG formation did not affect the stabilization of mRNAs, which also imply the existence of alternative factors [33].
Interesting mutations that prevented tetracycline transport would thus manifest themselves as not growing on tetracycline-containing media.
Smad7 mutations that prevented its binding to either HDAC-1 or E2F-1 resulted in a significant decrease in Smad7-mediated inhibition of cell proliferation.
Noteworthy of mention is that all transcripts of Glan contained mutations that prevented translation of transposase, however the consensus contig sequence that was assembled from a large number of EST from different cDNA libraries appeared intact.
Most of the A20 mutations found in Hodgkin's and mediastinal lymphomas were nonsense or frameshift mutations that prevented production of full-length A20 protein (Chanudet et al. 2009; Honma et al. 2009; Schmitz et al. 2009).
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That is achieved by acquiring mutations that prevent the shortening of the ends of chromosomes, or telomeres.
To confirm their hunch, the researchers attempted to induce tolerance in two strains of transgenic mice, both of which have mutations that prevent T cell apoptosis, or programmed cell death.
These cells, which form in the bone marrow, contain mutations that prevent them from differentiating into specialized blood-cell types.
One possibility is that survival of oncoprotein-transformed cells requires second mutations that prevent senescence or apoptosis.
Recessive mutations that prevent 3-hydroxyproline formation in type I collagen have been shown to cause forms of osteogenesis imperfecta.
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