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We engineered mutations that affected receptor-binding, G186V or L194P, into the E611 HA gene.
We chose Hhat constructs with mutations that affected PAT activity without compromising stability.
Mutations that affected the ability to elongate the infective filament at normal speed or that produce bipolar filamentation have been previously described in U. maydis.
The positive correlation between mutations that affected neurotransmitter release in mammalian cells [29] and in C. elegans indicates these mutations have similar functional effects for both orthologous proteins.
Similarly, none of the Y15A, K186Q, or LL241,242AA mutations that affected the intracellular interaction of HIV-1 IN and SIP1 (Figure 1B) affected the interaction in vitro (Figure S2).
Except for the FGFR2 and PIK3CA mutations that affected human tumour cell lines, the remaining 8 mutations were found in samples derived from primary tumours and were somatic in origin as assessed by the sequencing of the matched normal DNA (data not shown).
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Schüpbach's work also explores mutations that affect the embryo.
Mutations that affect the retromer pathway have also been found in familial Parkinson's disease.
At the DNA level there may be many different mutations that affect gene-expression levels, but very few potentially beneficial mutations that directly affect protein function.
Because most random mutations that affect intelligence will reduce it, evolution will tend to select against them.
From our cohort of 826 patients with ID, we selected all de novo mutations that affect mTOR-related genes.
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