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Mutations shown in the table are more than the number of isolates that were sent for sequencing.
Two other tables provide a summary of the statistics in COSMIC for the selected gene and a summary of the mutations shown in the figure.
Several of the mutations shown in figure 4 were consistently present at or above the 1% abundance, without being eliminated by other fluctuations in the population.
Munc13-KR/AA mutant was generated using the Quickchange kit to introduce the two point mutations shown in Figure 3 into knockdown resistant Munc13-1.
We sequenced all the control variants and damaging mutations shown in Figures 1 and 2, as well as additional case variants with less than 50-fold coverage.
As summarized in Fig. 3AB, mutations shown in green (Y61A, H47A, R159A, H259A, H281A, R304A, and F332A) had neither an effect on dimerization, autoproteolysis nor substrate cleavage.
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The pUL31 Y34andnd pUL34 L167A mutations showed, in this respect, a less severe phenotype.
Intriguingly, the N451D mutation (shown in blue in Fig. 7a) alone decreased viral transduction of CHO and Pro5 cells (Fig. 6), but when this mutation is combined with the D532N change, viral transduction of CHO cells is synergistically improved.
p.V600E mutation (control, shown in red) can be clearly distinguished from p.V600K mutation (shown in green) and p.V600R (shown in blue).
The decreased sucrose utilization by deletion of cra in EMY-69 was partially relieved by scrR mutation, shown in EMY-70 (Fig. 2c).
Sequencing analysis revealed that each mutant contains several mutations as shown in Fig. 1-D.
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