Deletion mutations, removing the zinc motif, the acid domain, or both domains, were engineered into EMCV cDNAs.
Splicing reporter assays demonstrated that one of the disease-causing mutations removes the uridine tract from Alu elements, which is required for hnRNP C binding.
As shown by recent heterologous expression studies, mutations removing the positive charges of the CBR3 loop lead to a loss of membrane localization of PTEN in living cells.
A point mutation removed the sensitivity to ruthenium red (RuR).
For example, the hokE_large mutation removes the fep/ ent operons, and this mutation associates with both pc2 and pc5 (Table 2).
The AKAP150ΔPIX mutation, removing the PIAIIIT motif (a.a. 655 661), was introduced into the mouse genome by homologous recombination (see Supplementary data).
The molecular modeling of the interaction between LVX and the DNA-DNA gyrase complex indicates that the loss of an acetyl group in the Asp94Gly mutation removes the acid base interaction with LVX necessary for the quinolone activity.
However, changing these residues in the B. taurus subunit (Gln, Asp, Glu and Asn) to their equivalent residues from the E. coli sequence (or, for the E143Q mutation, removing the charge) did not change the cluster potential significantly.
The remaining chemically induced mutations remove splice sites in the nascent dumpy message.
New fragments can also form via point mutation if the mutations remove (or create) a restriction site.
