Using eT1, the forward mutation frequency for MMC was observed to be 2% with an equal number of lethal mutations recovered in the first and second broods (A. M. Rose, unpublished results).
A second class of mutations, recovered only during evolution in higher sublethal concentrations of the antibiotic, deleted the C-terminal end of the ATP synthase shaft.
Mutations recovered in genes encoding elongation factors have revealed their involvement in a diverse range of biological pathways including the pathogenesis of numerous cancers, embryonic patterning, haematopoiesis, and also neuronal development, migration and degeneration.
Indeed, 62% of mutations recovered in this C. elegans screen are predicted to have an effect on the protein product (Table 2), although long-term studies are required to determine exactly how many of these mutations will result in a mutant phenotype.
The three modifier mutations recovered in msl1 cluster around the PEHE domain.
Our phenotypic characterization of all mutations recovered from the screen allowed us to set priorities for further study.
It is particularly interesting that spontaneous mutations recovered at the homeotic genes were due to transposon insertions (Bender et al. 1983).
Top and bottom panels show respectively the dinucleotide context of the mutations recovered from the conversion of T/G to T/A on the top strand or bottom strand of FM1.
The two A. thaliana TILLING populations, L er and Col, also differ in the proportion of heterozygous:homozygous mutations recovered in TILLING analyses, L er showing substantially higher total average ratio than Col (3.7 versus 2.1, respectively) [ 10].
Of the 26 mutations recovered, nearly all (92%) were GC to AT transitions at CpG sites, supporting previous data with regard to p53 mutations in Barrett's tumours (Gleeson et al, 1995, 1998).
