Sentence examples for mutations of interest from inspiring English sources

We used site-directed mutagenesis to generate mutations of interest in plasmid DNA derived from common bacterial artificial chromosome sources containing the cystic fibrosis transmembrane conductance receptor gene.

The paucity of clinically derived mutation-positive samples could be surmounted if it were possible to construct artificial samples containing mutations of interest that would sufficiently resemble natural human samples.

This is largely because mutations of interest are introduced into endogenous genes, just as they occur in the real patients, and because they are based on human genomes rather than rat genomes.

Second, most mutations of interest occur outside the sequence cleaved by a single restriction enzyme.

Control sequences, comprising all BCO and RCO binding sequences in the consensus subtype B HIV-1 pol, were constructed by ligating 10 short (<100 nt) oligonucleotides (Table S6) with degenerate positions corresponding to the mutations of interest (Figure 3A).

After recombination cloning into the pDONR221 vector, several entry clones were sequenced and the two active site mutations of interest defined as mut1 and mut2 were selected for further studies.

Similarly, it is possible to generate genetic mutant mice in which the genetic mutation of interest (a knocked-out, overexpressed, or mutated endogenous gene) is induced selectively within particular brain regions in adult animals.

In the resulting engineered cells, isolated functional measurement of the remaining allele carrying the rare mutation of interest is possible.

MARCM females were crossed to males carrying the mutation of interest.

In this case, only marked F2 animals are scored, and these should all be homozygous for the mutation of interest.

Phylogenetic analysis was performed on the group of pyrosequencing reads, containing the TDR mutation of interest, in a background of the 96 sequences obtained from Sanger sequencing.