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In combination, these tools will allow isolation of a series of mutations of differing strengths and types.
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This study further suggests that the rate differential between the first one or two with the remaining cell divisions can differ for mutations of different nature (here different lethalities).
ENU mutagenesis is another powerful technique that can be used to produce allelic series of mutations with differing effects upon function of single genes (e.g., Andrews et al., 2012).
Moreover, stability changes of mutations differing only in temperature and pH were highly correlated in the extracted data set.
However, neither the percentage of genomic aberrations, nor number of mutations differed among the tumor stages.
Moreover, the spectrum of mutations differed between sporadic colon carcinoma, where the predominant mutation was a G to A transition, and pancreatic carcinomas, which predominantly contained G to C or T transversions.
By examining all combinations of heterozygous and homozygous mutations, they demonstrate differing sensitivities of splicing events to the ESRP proteins.
The incidence of mutations differed significantly between HBC (14/60; 23.3%) and HBOC (19/32; 59.4%) families (p = 0.0006).
Thus, it appeared that the distribution of beneficial mutations differed in overall frequency of adaptive mutations between the resource treatments, such that more mutations were available in the benign environment.
These preliminary data suggest that the spectrum of mutations identified differs little from that seen in other studies, with no recurrent mutation and the mutations spread throughout the genes.
This approach has allowed us to identify several competing lines carrying different combinations of polymorphic mutations which differ in their fitness values and in their ability to fix when present in a simpler mutant spectrum.
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