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TP53 mutations occurred more often in basal-like lines (P = 0.038).
Assuming that recombination events or mutations occurred more frequently at coa loci than at flanking regions, we tried to detect recombination event using RDP3 program with sets of 28 nucleotide sequences of approximately 5.7 kb DNA region.
The present analysis showed that HPN-mediated frameshift mutations occurred more common in the genes that contained high content of HPN, principally including the genes involved in restriction and modification system, membrane-associated proteins, lipopolysaccharides and some hypothetic proteins (Table S3 and S4).
Regarding ethnicity, EGFR mutations occurred more frequently in Asian than in Western patients [20].
Therefore, we propose that, in LCT-KP289, mutations occurred more frequently in genes related to environmental adaptation.
On the other hand, although non-synonymous TP53 mutations occurred more frequently, we observed no correlation between these mutations and clinical outcome (data not shown).
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From this data set, we cannot determine why some of these "hotspots" are mutated and others are not, or why some mutations occur more frequently.
The gene encoding tumor suppressor p53 is commonly mutated in cancers, and contrary to the EGFR mutation, p53 mutations occur more frequently in smokers than in non-smokers (Ryberg et al., 1994; Garinis et al., 2001; Pfeifer et al., 2002).
Lastly, despite some data indicating no association between cytogenetics and TET2 status in MPN, TET2 mutations occur more often in the presence of normal karyotype and intermediate-risk AML (Hussein et al., 2010; Weissmann et al., 2012).
PIK3CA mutations occur more frequently in elder patients for HER2-positive breast cancer.
In wild-type yeast cells, mutations occur more frequently in old than in young cells (McMurray & Gottschling 2003).
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