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Finally, we introduced two mutations not reported to be related to fALS.
We identified 17 mutations not reported earlier (Table 2).
Rut-C30 had four additional mutations not reported earlier.
Novel mutations not reported in the database of COSMIC were confirmed by Sanger sequencing or pyrosequencing as described previously [ 12].
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In lung cancer, although the frequency of KRAS mutations is similar to that in colorectal cancer, these mutations are not reported to be a predictive marker.
As these mutations were not reported in earlier studies, we further characterized the effect of these mutations on the protein function and stability utilizing computational methods such as SIFT, Polyphen, and MD simulations.
These point mutations were not reported in the Leiden Muscular Dystrophy Database (http://www.dmd.nl/), which catalogs LMNA mutations associated with muscular dystrophy.
ARG1 splicing mutations are not reported often, and they are probably related to a more severe phenotype than missense mutations.
Unlike PORCN, WLS-coding region mutations are not reported to date in any human diseases.
The five mutations were not reported in the SNP databases (http://www.ncbi.nlm.nih.gov/SNP).nih.gov/SNP
However, cell sorting based on these mutations was not reported (Kirn et al., 2000).
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