Sentence examples for mutations not introduced from inspiring English sources

Specific changes present in all these CBMs included mutations not introduced in the design of the library from which the specific clones were selected.

The pattern of the mutations suggests that the majority of the mutations are not introduced from reactive oxygen species, but from the errors the mitochondria themselves make in the process of duplicating their DNA when a cell divides.

Two separate PCR reactions were done for each flaA allele, and were sequenced independently to ensure that mutations were not introduced during PCR.

Plasmids constructs were sequenced to ensure that unwanted mutations were not introduced by PCR and the cloning procedures.

If a mutation is not introduced, the target site is again targeted by CRISPR/Cas9, and once mutations are introduced the region is no longer targeted because of the modification of the target DNA sequence.

Although absence of the R46X mutation in the EBV-transformed lymphoblastoid cell lines strongly suggests that the mutation is not introduced during in vitro amplification, to further rule out this possibility, we also tested wild-type SDHB plasmids.

This suggests that mutation artefacts were not introduced during the methodology and that all mutations subsequently detected were genuinely present in the Barrett's tissues.

We also determined the structure of the isolated Btk kinase domain with the six mutations described above and found by comparing it to wild-type Btk that the mutations do not introduce major perturbations in the structure.

Therefore, these mutations do not introduce a frame shift into the coiled coil motif.

The slightly, but not dramatically, reduced enzyme activity of the K216A mutant indicates that a single point mutation did not introduce global conformational changes into the N-lobe, and confirmed the structural finding that Lys216 participates in phosphate binding.

This indicates that the mutations are present in genes and not introduced in the steps that produce mRNAs.