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Pools containing putative mutations must be deconvoluted in a second TILLING assay to identify the mutated individual.
"The timing of mutations must be earlier than the separation of populations," says Stanford geneticist Luca CavaLucaSforza.
For GR2 to evolve back into GR1, these five mutations must be reversed first to avoid this molecular fender-bender.
The differences both between species and in underlying causative mutations must be considered in choosing the best animal for a specific scientific study.
This perspective supports the emerging recognition that the long-standing paradigm of cancer origination from isolated genetic mutations must be supplanted by an enlarged one in which the concerted action of dysregulated oncoproteins overwhelm cellular defenses against cancer proliferation.
However, they do imply that any difference between these two types of mutations must be minor.
Similar(21)
The mutated ABCB7 gene is on the X chromosome; in males (XY), one copy of the mutated gene is sufficient to cause the disorder, while in females (XX), a mutation must be present in both copies to cause the condition.
In contrast, the process of gene identification is far more laborious in forward genetic screens since a causative (usually point) mutation must be identified amongst the entire genome.
These cysteine residues are in pairs at the protein level (Figure 2), but gene mutation must be at the DNA level where the corresponding regions of the two cysteine residues are quite far apart (Figure 1).
The mutation must be functionally relevant.
Thus, in spinach, the feminizing mutation must be in the suppression of B class expression.
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