Sentence examples for mutations made to from inspiring English sources

Nonintegrating lentiviral (NIL) vectors were produced from HIV-1-based lentiviral vectors by introducing combinations of mutations made to disable the integrase protein itself and to alter the integrase recognition sequences (att) in the viral LTR.

As a result, the mutations made to the polypyrimidine tract overlap the branch point C consensus sequence by one nucleotide.

To explore whether the mutations made to the C-terminus protein sequence produced a change in secondary structure, circular dichroism was performed on all C-terminal variants (data not shown).

Further evidence of the di-arginine motif binding to coatomer protein was provided more recently by key mutations made to the α and β subunit of COP I, which although led to the formation of the coatomer complex had lost its ability to bind to the di-lysine motif [29].

In contrast, out of the 16 mutations made to charged/polar residues (to lysine, glutamine, or glutamic acid), only six of these showed a complete loss in activity.

Schematic representations of 3′UTRs of cytoskeletal genes (A ) Cfl2, (B ) Wasl, (C ) Bod1, Twf1, (E ) Itgav, (F ) Grfl1 with miR-142-3p miR-142-3p miR-142-3p corresponding mutations made to test direct interactions with miR-142-3p.

Most of the other mutations studied have only been observed in low numbers of PD patients and further work is required to assess the contributions these mutations make to the development of disease [ 6].

(A ) Diagram of the mutation made to the BH3 domain of human Bcl2 to abolish BH3 domain function in the Bcl2 BH3 mutant transgenic (BH3 Tg) mice.

To dissect the contributions that each mutation makes to the structure of CDK9/cyclin TQ77R/E96G/F241L, we crystallized a complex that is wild-type at cyclin T positions Gln77 and Glu96 but retains the F241L mutation to aid crystallization.

A number of random mutations are made to the initial protein sequence and these mutations are evaluated for improvements of a specific trait.

Mutants with multiple mutations were made to screen TEN domain residues more efficiently, or to examine the compound effects of single mutations.