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The goals of this study were to evaluate the prognostic utility of histologic grade, c-KIT mutations, KIT staining patterns, and the proliferation markers Ki67 and AgNORs in dogs postoperatively treated with vinblastine and prednisone +/- RT, and to compare the outcome of dogs treated with post-operative chemotherapy +/- RT to that of a prognostically matched group treated with surgery alone.
This study confirms the prognostic value of histologic grade, c- KIT mutations, KIT staining patterns, and proliferation analyses for canine MCT.
In this study, histologic grade, the presence of c-KIT mutations, KIT staining patterns, and proliferation markers, Ki67 and AgNORs, were associated with the prognosis of MCT postoperatively treated with vinblastine and prednisone.
While there is much less clinical data, most of the more rare mutations, KIT exons 13 and 17, and PDGFRα exons 12 and 14, are sensitive to imatinib in vitro [ 9].
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With the CE-IVD-marked TheraScreen K-RAS Mutation kit, the mutational status of 89 of the 100 samples could be assessed in the initial analysis.
The mutants NCaBD E31Q), NCaBD E63Q) and NCaBD E31Q/E63Q) were created using the PFusion Mutation kit (New England Biolabs, Ipswich, MA).
which independently generated data by one of RASKET KIT, DS, and TheraScreen® K-RAS Mutation Kit (TheraScreen Kit), respectively.
The mutations were generated using a site-directed mutagenesis method (Quick-change Constructs mutation kit, Stratagene).
KRAS mutations were investigated using the TheraScreen K-ras mutation kit (Qiagen).
The mCRC samples were analyzed using the TheraScreen® KRAS mutation kit (Qiagen).
One of the most frequently used kits is the LightCycler® ApoE Mutation Kit by Roche Diagnostics (Basel, Switzerland).
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